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Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 265 kDa                                                          Uniprot ID: Q8NG31

Background:

Cancer susceptibility candidate gene 5 protein (CASC5) is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. CASC5 functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues. This gene was originally identified as a fusion partner with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14). Mutations in this gene cause autosomal recessive primary microcephaly-4 (MCPH4).

Other Names:

AF15Q14, CT29, D40, hKNL-1, hSpc105, KNL1, PPP1R55, Spc7, Protein CASC5, ALL1-fused gene from chromosome 15q14 protein, Bub-linking kinetochore protein, Blinkin, Cancer susceptibility candidate gene 5 protein, Cancer/testis antigen 29, Kinetochore-null protein 1, Protein D40/AF15q14, KIAA1570, KNL1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human CASC5. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Species Specificity:

Human

Product Data: