R2972-1 - RFX1 (N) Antibody, Rabbit Polyclonal

Catalog No. R2972-1
Product Name RFX1 (N) Antibody, Rabbit Polyclonal
Price $249

Quantity: 100 ul                                                                             Application: WB, IP, ChIP

Predicted | Observed M.W.: 105 I 135 kDa                                     Uniprot ID: P22670


Regulatory factor X 1 (RFX1) is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX1 is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. RFX1 binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat required for expression of hepatitis B virus genes [provided by RefSeq].

Other Names:

MHC class II regulatory factor RFX1, Enhancer factor C, EF-C, Regulatory factor X 1, RFX, Transcription factor RFX1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human RFX1. Antibodies were purified by affinity purification using immunogen.

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:


Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP & ChIP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

RFX1 Western IP ChIP Antibody (Abiocode)

Fig 1. (A) Western blot of total cell extracts from a,c. human HeLa, b,d. human Jurkat; using 2 independent Abs against 2 distinct regions of human RFX1 at RT for 2 h. (B) Total extracts from human HeLa were immunoprecipitated (IP) with IgG or anti-RFX1 (N) (R2972-1) under the conventional IP conditions or cross-linked chromatin immunoprecipitation (ChIP) conditions; followed by WB with the same Ab and Rabbit IgG light chain-specific 2nd antibody.