Products

R1855-vp - RALBP1 (vPairTM) Antibodies

Catalog No. R1855-vp
Product Name RALBP1 (vPairTM) Antibodies
Price $249
  
 

Predicted I Observed M.W.: 76 I 100 kDa                                                   Uniprot ID: Q15311

Application: WB

Quantity:        50 ul RALBP1 (N) (R1855-1) Rabbit Polyclonal Antibody &

                      50 ul RALBP1 (C) (R1855-2) Rabbit Polyclonal Antibody


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

RALBP1 activates specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. RALBP1 also catalyzes transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon.

Other Names:

RalA-binding protein 1, 76 kDa Ral-interacting protein, Dinitrophenyl S-glutathione ATPase, Ral-interacting protein 1, RLIP1, RLIP76

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [RALBP1 (N) (R1855-1)] or the C-terminal [RALBP1 (C) (R1855-2)] region of human RALBP1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: 

RALBP1 Antibody Western (Abiocode)

Fig 1. Western blot of total cell extracts from
(A) mouse brain, (B, E) mouse thymus, (C, F)
human HeLa, (D, G) human Jurkat; using 2
 independent Abs against 2 distinct regions of
human RALBP1 at RT for 2 h.