R1979-3 - NPAS2 (M) Antibody, Rabbit Polyclonal

Catalog No. R1979-3
Product Name NPAS2 (M) Antibody, Rabbit Polyclonal
Price $249

Quantity: 100 ul                                                                                       Application: WB, CHIP

Predicted I Observed M.W.: 91 kDa                                                         Uniprot ID: P97460


Neuronal PAS domain-containing protein 2 (NPAS2) is a nuclear protein and a component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. BMAL1-NPAS2 heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited by a feedback loop mediated by PER, and also by CRY proteins.

Other Names:

Neuronal PAS domain-containing protein 2, Neuronal PAS2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the middle region of mouse NPAS2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

ChIP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

NPAS2 Western ChIP Antibody (Abiocode)

Fig 1. (A) Western blot of total cell extracts from human HeLa, using
anti-NPAS2 (M) (R1979-3) at RT for 2 h. (B) Total extracts from human
HeLa were immunoprecipitated (IP) with IgG or anti-NPAS2 (M) (R1979-3)
under the cross-linked chromatin immunoprecipitation (ChIP) conditions;
followed by WB with the same Ab at RT for 2 h.