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Quantity: 100 ul                                                                                 Application: WB

Predicted | Observed MW: 73 | 75 kDa                                              Uniprot ID: P04049

Background:

RAF proto-oncogene serine/threonine-protein kinase (RAF1) belongs to the protein kinase superfamily. It acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). It regulates Rho signaling and migration, and is required for normal wound healing.  Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) and LEOPARD syndrome type 2 (LEOPARD2).

Other Names:

RAF proto-oncogene serine/threonine-protein kinase, c-Raf, cRaf, RAF, CRAF, Raf-1, Proto-oncogene c-RAF

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing the N-terminal region of human RAF1, and provided as whole antiserum. 

Storage Buffer and Condition:

Supplied as whole rabbit serum containing 30% Glycerol and 0.01% NaN₃. Store at -20oC. Stable for 6 months from date of receipt.

Species Specificity:

Human, mouse

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: