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R0267-1 - α-Tubulin (N) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB Predicted M.W.: 50 kDa Uniprot ID: Q71U36 Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. Tubulin A1A encodes alpha tubulin (a-Tubulin) and is highly similar to mouse and rat Tuba1 gene. Other Names: Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, TUBA1A, TUBA3 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human Tubulin A1A. The identical regions are also present in human Tubulin A1B and Tubulin A1C. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human Tested Applications: WB: 1:1,000-1:5,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications.
Product Data: Fig 1. Western blot of total cell extracts from (A) human HeLa, (B) human 293T, using Ab (R0267-1) at RT for 2 h.
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