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Predicted M.W.: 22 kDa                                                                                Uniprot ID: Q61337

Application: WB

Quantity:        50 ul BAD (N) (R0221-1) Rabbit Polyclonal Antibody &

                      50 ul BAD (C) (R0221-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^_TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Bcl2 antagonist of cell death (BAD) promotes cell death. BAD competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. BAD appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Other Names:

Bcl2 antagonist of cell death, Bcl-2-binding component 6, Bcl-xL/Bcl-2-associated death promoter, Bbc6

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [BAD (N) (R0221-1)] or the C-terminal [BAD (C) (R0221-2)] regions of mouse BAD. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Mouse

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: