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R3417-2 - RAD51 (C) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB Predicted I Observed M.W.: 37 kDa Uniprot ID: P94102
Background: RAD51 binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 is a component of the meiotic recombination pathway. RAD51 seems to play a role in mediating chromosome homology search, chromosome pairing and synapsis at early stages and probably chromosome crossing-over at later stages in meiosis. RAD51 is probably involved in the repair of meiotic double strand breaks (DBSs) generated by AtSPO11-1 and in homologous recombination. Its function is dispensable for vegetative growth and root mitosis. Other Names: DNA repair protein RAD51 homolog 1, Rad51-like protein 1, AtRAD51, At5g20850, F22D1.20 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana RAD51 (AT5G20850). Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Arabidopsis thaliana Tested Applications: WB: 1:500-1:2,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. Western blot analysis of total protein extractsfrom wild type arabidopsis leaves, untreated (Unt) orr-irradiated for 1 h (IR); using anti-RAD51 (C) (R3417-2)at RT for 2 h. The same filter was re-probed with annon-relevant Ab for the loading control.
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