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R0147-vp2 - FANCI (vPairTM) Antibodies
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Quantity: 50 ul FANCI (C2) (R0147-2) Rabbit Polyclonal Antibody & 50 ul FANCI (N) (R0147-3) Rabbit Polyclonal Antibody Product Introduction: vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Background: Fanconi anemia, complementation group I (FANCI) is required for maintenance of chromosomal stability. It plays a role in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. In response to DNA damage it is involved in the S phase and G2 phase checkpoints of the cell cycle. FANCI also promotes FANCD2 ubiquitination and recruitment to DNA repair sites. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage and defective DNA repair. Other Names: FLJ10719, KIAA1794, Protein FACI Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the C-terminal [FANCI (C2) (R0147-2)] or the N-terminal [FANCI (N) (R0147-3)] region of human FANCI. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human Tested Applications: WB: 1:1,000-1:5,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications.
Product Data: Fig 1. Western blot of total cell extracts from human HeLa, using Ab (A) R0147-2 or (B) R0147-3 at RT for 2 h.
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