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Quantity: 100 ul                                                                                             Application: WB

Predicted I Observed M.W.: 60 kDa                                                            Uniprot ID: Q6P1J9

Application: WB

Quantity:        50 ul CDC73 (N) (R2724-1) Rabbit Polyclonal Antibody &

                      50 ul CDC73 (C) (R2724-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

CDC73 is involved in transcriptional and post-transcriptional control pathways. CDC73 is a component of the the PAF protein complex, which associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. CDC73 appears to facilitate the association of 3' mRNA processing factors with actively-transcribed chromatin. Mutations in CDC73 have been linked to hyperparathyroidism-jaw tumor syndrome, familial isolated hyperparathyroidism, and parathyroid carcinoma.

Other Names:

Parafibromin, Cell division cycle protein 73 homolog, Hyperparathyroidism 2 protein, C1orf28, HRPT2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [CDC73 (N) (R2724-1)] or the C-terminal [CDC73 (C) (R2724-2)] region of human CDC73. Antibodies were purified by affinity purification using immunogen. 

Species Specificity:

Human

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: