R0872-vp - ANAPC5 (vPairTM) Antibodies

Catalog No. R0872-vp
Product Name ANAPC5 (vPairTM) Antibodies
Price $249

Predicted I Observed M.W.: 85 I 100 kDa                                                   Uniprot ID: Q9UJX4

Application: WB

Quantity:        50 ul MLXIPL (N2) (R2556-2) Rabbit Polyclonal Antibody &

                      50 ul MLXIPL (C) (R2556-3) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.


Anaphase-promoting complex subunit 5 (ANAPC5) belongs to the APC5 family and is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Other Names:

Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [ANAPC5 (N) (R0872-1)] or the C-terminal [ANAPC5 (C) (R0872-2)] region of human ANAPC5. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

ANAPC5 Antibody Western (Abiocode)

Fig 1. Western blot of total cell extracts from (A) mouse
thymus, (B, C) human HeLa, (D) human Jurkat; using 2
independent Abs against 2 distinct regions of human
ANAPC5 at RT for 2 h.