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Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 103 kDa                                                          Uniprot ID: Q15424

Background:

Scaffold attachment factor B (SAFB) is a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). SAFB is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. SAFB is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. SAFB is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene [provided by RefSeq].

Other Names:

Scaffold attachment factor B1, SAF-B, SAF-B1, HSP27 estrogen response element-TATA box-binding protein, HSP27 ERE-TATA-binding protein, HAP, HET, SAFB1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human SAFB. Antibodies were purified by affinity purification using immunogen.

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: