R1086-4 - TET2 (N2) Antibody, Rabbit Polyclonal

Catalog No. R1086-4
Product Name TET2 (N2) Antibody, Rabbit Polyclonal
Price $279

Quantity: 100 ul                                                                              Application: WB, IP, ChIP, IF

Predicted I Observed M.W.: 224 kDa                                              Uniprot ID: Q4JK59


TET2 is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). 5-hydroxymethylcytosine may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation.

TET2 plays an important role in myelopoiesis, and defects in TET2 gene have been associated with several myeloproliferative disorders.

Other Names:

Methylcytosine dioxygenase TET2, KIAA1546

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of mouse TET2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:


Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP & ChIP: 1:100-1:200

IF: 1:100-1:300

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

TET2 Western Antibody IP ChIP (Abiocode)

Fig 1. A) Western blot of total cell extracts from human 293 T cells transfected with the empty vector, or Flag-tagged murine TET1, TET2 or TET3; using indicated Abs at RT for 2h. B) Western blot of total cell extracts from mouse MEF cells containing a control shRNA (a) or a shRNA specific for murine TET2 (b); using anti-TET2 (N2) (R1086-4) or anti-Tubulin (N) (R0267-1) at RT for 2h. C) Total extracts from mouse thymus were immunoprecipitated (IP) with IgG or 2 independent TET2 Abs as indicated under the conventional IP conditions or the cross-linked chromatin immunoprecipitation (ChIP) conditions; followed by WB with anti-TET2 (C2) (R1086-6b) at RT for 2 h.