Abiocode Logo

Predicted I observed M.W.: 74 I 110 kDa                                                      Uniprot ID: Q8BN78

Application: WB

Quantity:        50 ul KAISO (N) (R0175-1) Rabbit Polyclonal Antibody &

                        50 ul KAISO (N2) (R0175-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

KAISO is also known as Zinc finger and BTB domain-containing protein 33, a transcriptional regulator with bimodal DNA-binding specificity.  KAISO binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. KAISO can recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. In contrast, KAISO may also activate transcription of a subset of target genes by the recruitment of CTNND2.

Other Names:

ZBTB33, ZNF348, Zinc finger and BTB domain-containing protein 33

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing 2 distinct N-terminal regions of mouse KAISO. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.                   

Product Data: