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Predicted I Observed M.W.: 135 I 150-160 kDa                                         Uniprot ID: Q9BXA9

Application: WB

Quantity:        50 ul SALL3 (N) (R1429-1) Rabbit Polyclonal Antibody &

                       50 ul SALL3 (C) (R1429-2) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Sal-like protein 3 (SALL3) is a sal-like C2H2-type zinc-finger protein, and belongs to a family of evolutionarily conserved genes found in species as diverse as Drosophila, C. elegans, and vertebrates. Mutations in some of these genes are associated with congenital disorders in human, suggesting their importance in embryonic development. SALL3 binds to DNA methyltransferase 3 alpha (DNMT3A), and reduces DNMT3A-mediated CpG island methylation. It is suggested that silencing of SALL3, resulting in acceleration of DNA methylation, may have a role in oncogenesis [provided by RefSeq].

Other Names:

Sal-like protein 3, Zinc finger protein 796, Zinc finger protein SALL3, hSALL3, ZNF796

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [SALL3 (N) (R1429-1)] or the C-terminal [SALL3 (C) (R1429-2)] regions of human SALL3. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: